Thermodynamics of Binding Silver Ion to Jack Bean Urease
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چکیده
منابع مشابه
The nickel ion environment in jack bean urease.
Preliminary results of an extended X-ray absorption fine structure (e.x.a.f.s.) and X-ray absorption near edge structure study of jack bean urease have recently been reported [Hasnain & Piggott (1983) Biochem. Biophys. Res. Commun. 112, 279]. These results indicate that the environment of the nickel ion in the enzyme is similar to that in the model compounds Ni(L)2(L')1(ClO4)1 (where L is 1-n-p...
متن کاملKinetics and mechanism of jack bean urease inhibition by Hg2+
UNLABELLED BACKGROUND Jack bean urease (EC 3.5.1.5) is a metalloenzyme, which catalyzes the hydrolysis of urea to produce ammonia and carbon dioxide. The heavy metal ions are common inhibitors to control the rate of the enzymatic urea hydrolysis, which take the Hg2+ as the representative. Hg2+ affects the enzyme activity causing loss of the biological function of the enzyme, which threatens ...
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Investigation of their ultracentrifugal and electrophoretic behavior and chemical reactivity has been used to characterize the suprastructure of the nongenetic urease isozymes. As purified from jack bean meal, urease is obtained in the LY form (M, = 480,000, s20,W = 18.3), which is composed of two noncovalently bound, equal and active A1 half-units (M, = 240,000, sZo,W = 11.5) which separate af...
متن کاملThe amino acid composition of jack-bean urease.
Analyses were carried out on twice-recrystallized freeze-dried urease (Bailey & Boulter, 1969) that had previously been dialysed for 24hr. in the cold against running tap water, and then for 24hr. against three changes of deionized water. Acid hydrolysis of urease (5mg.) was carried out at 1100 in evacuated heavy-walled Pyrex tubes with threetimes-redistilled 5-7M-hydrochloric acid at a concent...
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Helicobacter pylori :28 µg/mL). (H. pylori) infection leads to different clinical and pathological outcomes in humans, including chronic gastritis, peptic ulcer disease and gastric neoplasia and even gastric cancer and its eradiation dependst upon multi-drug therapy. The most effective therapy is still unknown and prompts people to make great efforts to find better and more modern natural or ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2010
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2009.12.1305